Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cross-reactivity00:42

Cross-reactivity

Overview
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Affinity and Avidity01:41

Affinity and Avidity

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neurofilament Light Protein: A Marker for Injury Severity, Clinical Course, and Outcome Following Moderate to Severe Traumatic Brain Injury.

Neurocritical care·2026
Same author

Historical and contemporary chronic wasting disease prions from the western United States demonstrate similar strain properties.

The Journal of general virology·2026
Same author

Corrigendum to 'Role of CD40 in prion disease and the immune response to recombinant PrP' [Journal of Neuroimmunology 257 (2013) 21-27/475655].

Journal of neuroimmunology·2026
Same author

Discovery of Novel Isofunctional SARS-CoV‑2 NSP14 RNA Cap Methyltransferase Inhibitors by Structure-Based Virtual Screening.

ACS medicinal chemistry letters·2025
Same author

Discovery, Optimization, and Evaluation of Non-Nucleoside SARS-CoV-2 NSP14 Inhibitors.

Journal of medicinal chemistry·2025
Same author

Early cerebrospinal fluid elevations of pTau-217 in severe traumatic brain injury subjects.

Frontiers in neurology·2025

Related Experiment Video

Updated: Jun 28, 2026

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

PrP antibody binding-induced epitope modulation evokes immunocooperativity.

Binggong Chang1, Michael W Miller, Marie S Bulgin

  • 1Department of Biochemistry, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA.

Journal of Neuroimmunology
|November 4, 2008
PubMed
Summary

Researchers developed new prion protein (PrP) antibodies that reveal a phenomenon called positive immunocooperativity. This binding enhancement could improve diagnostic test sensitivity for prion diseases.

More Related Videos

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
11:10

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Published on: June 29, 2016

Related Experiment Videos

Last Updated: Jun 28, 2026

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
11:10

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Published on: June 29, 2016

Area of Science:

  • Immunology
  • Neuroscience
  • Biochemistry

Background:

  • Prion diseases are linked to misfolded prion proteins (PrP).
  • Understanding antibody-PrP interactions is crucial for diagnostics.
  • Existing methods for PrP detection often require harsh treatments.

Purpose of the Study:

  • To characterize antibody-antigen binding events for prion protein (PrP).
  • To investigate the impact of denaturation on PrP epitope accessibility.
  • To explore a novel phenomenon of enhanced antibody binding.

Main Methods:

  • Utilized three new prion protein-specific monoclonal antibodies (Mabs).
  • Assessed immunoreactivity under various denaturation conditions (heat, SDS).
  • Mapped antibody epitopes on the prion protein.

Main Results:

  • Heat and SDS denaturation maximized epitope accessibility and antibody binding.
  • Harsh denaturation did not fully abolish protein conformation.
  • Identified distinct PrP epitopes for different Mabs (e.g., PrP(93-122), PrP(155-200)).
  • Discovered positive immunocooperativity: one Mab binding enhances another's binding.
  • This effect is epitope-specific and sequence-dependent.

Conclusions:

  • Positive immunocooperativity enhances PrP detection sensitivity.
  • This phenomenon may eliminate the need for protease digestion in assays.
  • Potential for improved diagnostic assays for prion diseases like PrP(Sc).