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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

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Related Experiment Video

Updated: Jun 28, 2026

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
08:04

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

STEM tomography for thick biological specimens.

Kazuhiro Aoyama1, Tomoko Takagi, Ai Hirase

  • 1FEI Company Japan Ltd., Application Laboratory, NSS-II Building, 2-13-34 Kohnan, Minato-ku, Tokyo 108-0075, Japan. kazuhiro.aoyama@fei.com

Ultramicroscopy
|November 4, 2008
PubMed
Summary

Scanning transmission electron microscopy (STEM) tomography enables 3D analysis of thick biological specimens up to 1 micrometer. This advanced technique offers advantages over conventional transmission electron microscopy (TEM) with reduced specimen damage.

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Area of Science:

  • Electron microscopy
  • Biological imaging
  • Cell biology

Background:

  • Conventional transmission electron microscopy (TEM) faces challenges in imaging thick biological specimens.
  • Scanning transmission electron microscopy (STEM) tomography presents potential advantages for such samples.

Purpose of the Study:

  • To evaluate the efficacy of STEM tomography for 3D imaging of thick biological specimens.
  • To compare specimen damage between STEM and TEM tomography.

Main Methods:

  • Application of STEM tomography to yeast cells, HEK293 cells, and primary neurons embedded in resin.
  • Sectioning specimens into 1-micrometer-thick slices.
  • Utilizing a small condenser aperture for imaging thick specimens at large tilt angles (up to 73 degrees).

Main Results:

  • Successful 3D analysis of 1-micrometer-thick biological specimens using STEM tomography.
  • STEM tomography demonstrated effectiveness for thick specimens, overcoming limitations of conventional TEM.
  • Quantitative comparison revealed lower irradiation damage in STEM compared to TEM tomography.

Conclusions:

  • STEM tomography is a powerful tool for high-resolution 3D imaging of thick biological samples.
  • The technique offers significant advantages, including suitability for thicker specimens and reduced radiation damage.
  • STEM tomography represents an advancement for detailed ultrastructural analysis in cell biology.