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Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of microbial community structure in
Miho Uchida1, Haruna Hatayoshi, Aoi Syuku-nobe
1Department of Environmental Chemistry and Ecoengineering, Graduate School of Environmental Studies, Tohoku University, Sendai, Miyagi, Japan. miho@tohtech.ac.jp
Abstract:
The structures of microbial communities in water samples obtained from a landfill site that had been a source of environmental pollution by emitting hydrogen sulfide were elucidated using polymerase chain reaction-denaturing gradient gel electrophoresis. The microbial communities, which consisted of a limited number of major microorganisms, were stable for several months. Microorganisms capable of degrading such chemical compounds as 2-hydroxybenzothiazole and bisphenol A were observed in landfill leachate. Microorganisms responsible for the production of hydrogen sulfide were not the primary microbes detected, even in water samples obtained from the site of gas emission.
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