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Determination of amprolium in feed by a liquid chromatography-mass spectrometry method
S Squadrone1, C Mauro, G L Ferro
1C.Re.A.A. - National Reference Center for the Surveillance and Monitoring of Animal feed, c/o Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e valle D'Aosta, Via Bologna 148, 10154 Torino, Italy. stefania.squadrone@izsto.it
Abstract:
As a consequence of the finding of veterinarian drugs in food European Community banned several compounds like coccidiostats as amprolium (APL). This antibiotic has been used as a preventive and clinical anticoccidial drug in chicken. The 2005/187/CE, 2005/925/EC Recommendations ban the use of amprolium as additive in chicken feed. For this reason a rapid and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed to detect amprolium in chicken feed following the European community proposed technique (1999/27/EC) for sample preparation. Cause the validation is required for the analytical methods used in feed official control, this method was validated according to 2004/882/EC Regulation.
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