Fabrication of a highly sensitive penicillin sensor based on charge transfer techniques

Area of Science:

• Electrochemistry
• Biosensor Technology
• Analytical Chemistry

Background:

• Penicillin detection is crucial in pharmaceutical and food industries.
• Existing biosensors often face limitations in sensitivity and response time.
• The development of rapid, sensitive, and reliable penicillin detection methods is essential.

Purpose of the Study:

• To fabricate and demonstrate a highly sensitive penicillin biosensor.
• To utilize a charge-transfer technique (CTTPS) for signal amplification.
• To evaluate the performance of the CTTPS-based biosensor for penicillin detection.

Main Methods:

• Fabrication of a penicillin biosensor based on charge-transfer technique (CTTPS).
• Utilized a charge accumulation technique for penicilloic acid and H(+) ion perception.
• Employed charge accumulation cycles for signal amplification without external amplifiers.

Main Results:

• The CTTPS biosensor demonstrated high sensitivity (47.852 mV/mM) and a wide linear range (0-25 mM).
• Achieved a low detection limit of approximately 0.01 mM and a fast response time (<3s).
• Exhibited significantly higher sensitivity (nearly 8x) compared to the conventional ISFET penicillin sensor.

Conclusions:

• The developed CTTPS penicillin biosensor offers excellent performance, including high sensitivity and a low detection limit.
• CTTPS provides a viable alternative for amplifying sensing signals without external amplifiers.
• The sensor system is effective for measuring penicillin concentration in fermentation broths.