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Using multiplex real time PCR in order to streamline a routine diagnostic service
R N Gunson1, S Bennett, A Maclean
1West of Scotland Specialist Virology Centre, Gartnavel General Hospital, Glasgow G12 OYN, UK. rory.gunson@northglasgow.scot.nhs.uk
Summary
Multiplex real-time PCR assays streamline virology diagnostics. This paper details the development and benefits of these advanced in-house assays for routine laboratory services.
Area of Science:
- Virology
- Molecular Diagnostics
- Laboratory Science
Background:
- Real-time PCR assays are increasingly used in virology diagnostics.
- The expansion of available tests necessitates service rationalization through multiplexing.
Purpose of the Study:
- To describe the development of multiplex real-time PCR assays.
- To highlight challenges and benefits of implementing these assays in a routine service.
Main Methods:
- Development of qualitative and quantitative multiplex real-time PCR assays.
- Implementation into a routine virology testing service since 2003.
Main Results:
- Successful introduction of numerous multiplex real-time PCR assays.
- Identification of challenges encountered during assay development and implementation.
Conclusions:
- Multiplex real-time PCR assays offer significant benefits to routine virology diagnostic services.
- The development process provided valuable insights into optimizing multiplex assay implementation.
