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Updated: Jun 28, 2026

06:54
Injection of An. stephensi Embryos to Generate Malaria-resistant Mosquitoes
Published on: July 4, 2007
Injection of An. stephensi embryos to generate malaria-resistant mosquitoes
Olle Terenius1, Jennifer Juhn, Anthony A James
1Department of Molecular Biology and Biochemistry, University of California, Irvine, USA. olle.terenius@uci.edu
Journal of Visualized Experiments : Jove
|November 4, 2008
Abstract:
The introduction of exogenous genes into the genomes of mosquitoes requires microinjection techniques tailored to the specific species of interest. This video protocol demonstrates a method used by the James laboratory to microinject DNA constructs into Anopheles stephensi embryos for the generation of transformed mosquitoes. Techniques for preparing microinjection needles, collecting and preparing embryos and performing the microinjection are illustrated.

