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Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
Published on: July 4, 2007
Protocol for Plasmodium falciparum infections in mosquitoes and infection phenotype determination
Zhiyong Xi1, Suchismita Das, Lindsey Garver
1Malaria Research Institute, Bloomberg School of Public Health, John Hopkins University, USA.
Journal of Visualized Experiments : Jove
|November 4, 2008
Abstract:
Once a gene is identified as potentially refractory for malaria, it must be evaluated for its role in preventing Plasmodium infections within the mosquito. This protocol illustrates how the extent of plasmodium infections of mosquitoes can be assayed. The techniques for preparing the gametocyte culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.
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