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Updated: Jun 28, 2026
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Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)
Published on: July 4, 2007
Protocol for RNAi assays in adult mosquitoes (A. gambiae)
Lindsey Garver1, George Dimopoulos
1Malaria Research Institute, Bloomberg School of Public Health, John Hopkins University, USA.
Journal of Visualized Experiments : Jove
|November 4, 2008
Abstract:
Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the Dimopoulos lab to inject dsRNA into Anopheles gambiae mosquitoes, which harbor the malaria parasite. The technique manipulating the injection setup and injecting dsRNA into the thorax is illustrated.
