Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Studies on yeast peroxisomal citrate synthase.

G Kispal1, P A Srere

  • 1Pre-Clinical Science Unit, Veterans Administration Medical Center, Dallas, Texas 75216.

Archives of Biochemistry and Biophysics
|April 1, 1991
PubMed
Summary

Researchers purified peroxisomal citrate synthase (CS2) from yeast lacking mitochondrial citrate synthase (CS1). This purified CS2 enzyme exhibits distinct properties compared to CS1, including differences in purification, kinetics, stability, and membrane binding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Opposite regulation of uncoupling protein 1 and uncoupling protein 3 in vivo in brown adipose tissue of cold-exposed rats.

FEBS letters·2002
Same author

Synthesis and antibacterial study of unsaturated Mannich ketones.

European journal of medicinal chemistry·2001
Same author

An essential function of the mitochondrial sulfhydryl oxidase Erv1p/ALR in the maturation of cytosolic Fe/S proteins.

EMBO reports·2001
Same author

Mitochondrial ABC transporters.

Research in microbiology·2001
Same author

Isolation and subfractionation of mitochondria from the yeast Saccharomyces cerevisiae.

Methods in cell biology·2001
Same author

[Screening for carrier state of Haemophilia B using indirect genomic detection].

Orvosi hetilap·2001

Area of Science:

  • Biochemistry
  • Cell Biology
  • Yeast Genetics

Background:

  • Mitochondrial citrate synthase (CS1) is crucial for the citric acid cycle.
  • The existence and function of a peroxisomal citrate synthase (CS2) in Saccharomyces cerevisiae were previously suggested.
  • Understanding CS2 is important for comprehending cellular metabolic compartmentalization.

Purpose of the Study:

  • To purify and characterize the peroxisomal citrate synthase (CS2) from yeast.
  • To compare the biochemical and biophysical properties of CS2 with those of mitochondrial citrate synthase (CS1).
  • To investigate the distinct behavior of CS2 in yeast lacking CS1.

Main Methods:

  • Purification of CS2 from a Saccharomyces cerevisiae strain with a disrupted CS1 gene.

Related Experiment Videos

  • Enzyme kinetics assays.
  • Stability studies.
  • Analysis of binding to mitochondrial inner membranes.

    • Main Results:

    • CS2 was successfully purified from yeast lacking CS1.
    • Purified CS2 demonstrated different purification characteristics compared to CS1.
    • CS2 exhibited distinct kinetics, stability, and binding properties relative to CS1.
    • CS2 binds to the inner surface of mitochondrial inner membranes.

    Conclusions:

    • Peroxisomal citrate synthase (CS2) is a distinct enzyme from mitochondrial citrate synthase (CS1).
    • CS2 possesses unique biochemical and biophysical properties.
    • CS2's ability to bind to mitochondrial inner membranes suggests potential roles in metabolic crosstalk or regulation.