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Updated: Jun 28, 2026

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
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Ion channels modulating mouse dendritic cell functions.

Nicole Matzner1, Irina M Zemtsova, Thi Xuan Nguyen

  • 1Department of Physiology, University of Tübingen, Gmelinstr. 5, Tübingen, Germany.

Journal of Immunology (Baltimore, Md. : 1950)
|November 5, 2008
PubMed
Summary
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Lipopolysaccharide (LPS) activates dendritic cells (DCs) by increasing intracellular calcium via calcium release-activated calcium (CRAC) channels. This calcium influx is crucial for DC maturation and function, influencing immune responses.

Area of Science:

  • Immunology
  • Cell Biology
  • Signal Transduction

Background:

  • Calcium (Ca2+)-mediated signal transduction is vital for dendritic cell (DC) responses to antigens.
  • Mechanisms of increased intracellular calcium ([Ca2+]i) during DC activation were previously unclear.

Purpose of the Study:

  • To elucidate the mechanisms of Ca2+ influx in lipopolysaccharide (LPS)-stimulated DCs.
  • To investigate the role of Ca2+ channels and potassium (K+) channels in DC activation and function.

Main Methods:

  • Whole-cell voltage-clamp electrophysiology to study LPS-induced currents in mouse DCs.
  • Measurement of intracellular calcium ([Ca2+]i) changes.
  • Assessment of DC maturation and function markers (MHC class II, CCL21 migration, cytokine production, phagocytosis) under pharmacological inhibition.

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Main Results:

  • LPS treatment rapidly increased [Ca2+]i in DCs through intracellular store release and extracellular influx.
  • LPS-induced currents resembled those of Ca2+ release-activated Ca2+ (CRAC) channels, showing high Ca2+ selectivity and inward rectification.
  • LPS-induced [Ca2+]i increase was sensitive to Kv channel inhibitors (margatoxin, ICAGEN-4) and an I(CRAC) inhibitor (SKF-96365).
  • Inhibition of Ca2+ influx and Kv channels altered DC maturation and function, decreasing MHC class II expression, CCL21-dependent migration, and TNF-alpha/IL-6 production, while increasing phagocytic capacity.

Conclusions:

  • Ca2+ influx in LPS-stimulated DCs occurs via CRAC channels and is modulated by Kv channel activity.
  • This Ca2+ influx is essential for key dendritic cell maturation and functions, impacting immune responses.