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Updated: Jun 28, 2026

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
11:49

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

Published on: August 21, 2018

Characterization of RNA-protein interactions by phosphorothioate footprinting and its applications to the ribosome

A Ozlem Tastan Bishop1, Ulrich Stelzl, Markus Pech

  • 1Department of Biochemistry, University of Pretoria, Pretoria, South Africa.

Methods in Molecular Biology (Clifton, N.J.)
|November 5, 2008
PubMed

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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...