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Updated: Jun 28, 2026
Identification of protein complexes with quantitative proteomics in S. cerevisiae
Published on: March 4, 2009
The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins
Jane E Jackman1, Elizabeth J Grayhack, Eric M Phizicky
1Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York, USA.
Abstract:
Biochemical assay of proteomic libraries derived from the Saccharomyces cerevisiae genome provides a powerful new tool for the assignment of activities to proteins. Particular advantages of this approach include the speed with which a protein can be identified and the generality for any biological activity for which an assay can be developed. We discuss the utility of this approach for the identification of RNA-modifying enzymes using a yeast proteomic library derived from a genomic set of strains expressing GST-ORF fusion proteins. This technique is also broadly applicable to other classes of RNA-protein interactions, including RNA binding and RNA degradation, and can be used with any of the proteomic libraries that are available.
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