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Antioxidant effect ofSalvia miltiorrhiza
H S Kang1, H Y Chung, J H Jung
1Department of Food and Life Science, Pukyong National University, 608-737, Pusan, Korea.
Archives of Pharmacal Research
|November 5, 2008
Summary
The methanol extract of Salvia miltiorrhiza Bunge demonstrated significant antioxidant activity. Two isolated compounds, dimethyl lithospermate and 3-(3,4-dihydroxyphenyl)lactamide, showed superior radical scavenging effects compared to L-ascorbic acid.
Area of Science:
- Pharmacology
- Natural Products Chemistry
- Biochemistry
Background:
- Salvia miltiorrhiza Bunge (Labiatae) is a traditional medicinal herb.
- Antioxidants play a crucial role in combating oxidative stress.
- Identifying novel antioxidant compounds from natural sources is of significant interest.
Purpose of the Study:
- To isolate and identify antioxidant compounds from the methanol extract of Salvia miltiorrhiza.
- To evaluate the antioxidant potential of isolated compounds.
- To assess the cytoprotective effects of these compounds.
Main Methods:
- Activity-directed fractionation of Salvia miltiorrhiza methanol extract.
- Radical scavenging assay using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical.
- Spectroscopic data analysis for compound identification.
- Dichlorofluorescein (DCF) method for free radical generation inhibition.
- Cytoprotective assay against tert-butyl hydroperoxide (t-BHP) in cultured liver cells.
Main Results:
- A methanol extract of Salvia miltiorrhiza exhibited strong antioxidant activity.
- Two compounds, identified as dimethyl lithospermate and 3-(3,4-dihydroxyphenyl)lactamide, were isolated.
- Both isolated compounds demonstrated superior DPPH radical scavenging activity compared to L-ascorbic acid.
- These compounds also showed significant inhibition of free radical generation and cytoprotective effects.
Conclusions:
- Dimethyl lithospermate and 3-(3,4-dihydroxyphenyl)lactamide are potent antioxidant principles from Salvia miltiorrhiza.
- These compounds possess significant radical scavenging and cytoprotective properties.
- Salvia miltiorrhiza is a valuable source of novel antioxidants with potential therapeutic applications.