Related Experiment Video
Updated: Jun 28, 2026

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
Published on: March 6, 2015
[Influence of Wnt-1 recombinant adenovirus on differentiation of human epidermal stem cells]
Yuan-chao Li1, Jin-jin Wu, Jian-jun Bi
1Department of Dermatology, Daping Hospital, Field Surgery Institute, the Third Military Medical University, Chongqing 400042, PR China.
Objective:
To observe the influence of Wnt-1 recombinant adenovirus on differentiation tendency of human epidermal stem cells.
Methods:
Wnt-1 recombinant adenovirus was transduced into hESCs (E group), while normal hESCs were used as control (C) group. The diameter, proliferation,and labeling molecular expression of hESC were determined. The content of MMP-2 and MMP-7 in supernate were also assayed.
Results:
There was no obvious difference in diameter of hESC between two groups. The density of hESC in E group was (1.45 +/- 0.09) x 10(5)/mL, which was obviously higher than that in C group [(1.18 +/- 0.10) x 10(5)/mL, P < 0.05]. There were no obvious differences in expression of markers between two groups,including keratin 5 (KS), K6, K7, KS, K14, CD44, carcinoembryonic-like antigen (CEAA), ER, PR (P > 0.05) ,while the expression of K 10 was different among groups [(60 +/- 3)% in E group, 0 in C group], also K18 [(34.3 +/- 2.1)% in E group vs. (13.8 +/- 1.7)% in C group, P < 0.05], and K19 [(17.1 +/- 1.8)% in E group vs. (24.4 +/- 1.5)% in C group, P < 0.05].The contents of MMP-2 and MMP-7 in E group were higher than those in C group (P < 0.01).
Conclusion:
Wnt-1 recombinant adenovirus can induce the differentiation of hESCs to glandular epithelium-like cells.
More Related Videos
11:13Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells
Published on: April 7, 2016
15:33Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
Published on: August 13, 2013
Related Concept Videos
Renewal of Skin Epidermal Stem Cells
Clinical Applications of Epidermal Stem Cells
Role Of Notch Signalling In Intestinal Stem Cell Renewal
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal