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The effect of volume of chorionic villi on long-term cell culture
Min Chen1, Lucy Ng, Elizabeth Lau
1Department of Obstetrics and Gynaecology, The University of Hong Kong, Queen Mary Hospital, Hong Kong, SAR, China. drchenmin2003@yahoo.com.hk
Objective:
It was the aim of our study to investigate the association between culture time and weight of villi obtained by transabdominal chorionic villus sampling (CVS).
Methods:
We analyzed 1,442 villus samples.
Results:
The gestational age at sampling ranged from 10 to 14 weeks. The weight of villi in these samples ranged from 1 to 80 mg (median 10 mg, interquatile range 7-12 mg). The culture time ranged from 5 to 24 days. Culture time was significantly and inversely correlated with the weight of villi obtained (r = -0.258, p < 0.01). Time was significantly longer when 5 mg or less of villi was obtained but the difference was only up to 2 days.
Conclusions:
Because the difference in culture time for different groups of villus samples was within 1-2 days, this relationship is statistically significant but has no clinical significance.
