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Updated: Jun 28, 2026
Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants
Published on: January 26, 2019
Introducing pulsed low-intensity ultrasound to culturing human umbilical cord-derived mesenchymal stem cells
Jong Hyun Yoon1, Eun Youn Roh, Sue Shin
1Department of Laboratory Medicine, Seoul National University Boramae Hospital, 425 Shindaebang-dong, Dongjak-gu, Seoul, 156-707, South Korea.
Abstract:
The human umbilical cord (hUC) is a source of adult tissue-derived mesenchymal stem cells (MSCs). A pulsed low-intensity ultrasound (PLIUS) method is described for increasing the yield of MSCs from whole hUC without enzymatic digestion or growth factor supplementation. Analysis of the immunophenotype of cells and a differentiation study were performed to show the compatibility of MSCs. The mean number of cells recovered from primocultures of hUC was 6 x 10(5) cells/cm. PLIUS resulted in a 3.3-fold increase in MSC yield at passage 0. PLIUS exposure increases the yield of hUC-MSCs by promoting release and enhancing proliferation.
