Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 28, 2026

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping
09:43

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Published on: March 20, 2017

Simultaneous LIF and retro-reflected beam interference detection for CE.

Bo Xiong1, Wei Wang, Xueyan Miao

  • 1College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, PR China.

Electrophoresis
|November 6, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated physiological, hormonal, and transcriptomic analyses reveal pistil responses to pollen sources of varying compatibility in 'Fengtang' plum (Prunus salicina).

BMC plant biology·2026
Same author

Author Correction: Gut microbiota-modulated glutamic acid rejuvenates the quality of oocytes deteriorated by advanced reproductive age.

EMBO molecular medicine·2026
Same author

Gut microbiota-modulated glutamic acid rejuvenates the quality of oocytes deteriorated by advanced reproductive age.

EMBO molecular medicine·2026
Same author

Uridine restores oocyte quality and mitigates female reproductive aging by inhibition of ferroptosis in mice.

Nature communications·2026
Same author

Curtain grouting volume prediction using a Bayesian-optimized stacking ensemble model with SHAP analysis.

Scientific reports·2026
Same author

Diisocyanate-mediated covalent grafting of lignin onto chitosan enables hyperconjugation-driven dramatic enhancement in methylene blue adsorption.

International journal of biological macromolecules·2026
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

A new optical detection method combines laser-induced fluorescence (LIF) and retro-reflected beam interference for capillary electrophoresis (CE). This approach offers high sensitivity and broad applicability for analyzing diverse samples without pretreatment.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Optical Detection Techniques

Background:

  • Capillary electrophoresis (CE) is a powerful separation technique.
  • Existing detection methods for CE often have limitations in sensitivity or universality.
  • Optical detection methods, such as laser-induced fluorescence (LIF), are widely used but can be enhanced.

Purpose of the Study:

  • To develop a novel, on-column optical detection method for capillary electrophoresis.
  • To combine laser-induced fluorescence (LIF) detection with retro-reflected beam interference detection.
  • To evaluate the performance of this dual-mode detection system for analyzing complex samples.

Main Methods:

  • A side-illuminated laser beam was employed for on-column detection in CE.

More Related Videos

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

Measurement of Quantum Interference in a Silicon Ring Resonator Photon Source
12:19

Measurement of Quantum Interference in a Silicon Ring Resonator Photon Source

Published on: April 4, 2017

Related Experiment Videos

Last Updated: Jun 28, 2026

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping
09:43

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Published on: March 20, 2017

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

Measurement of Quantum Interference in a Silicon Ring Resonator Photon Source
12:19

Measurement of Quantum Interference in a Silicon Ring Resonator Photon Source

Published on: April 4, 2017

  • The system integrated laser-induced fluorescence (LIF) and retro-reflected beam interference detection.
  • Key parameters of the optical detection scheme were optimized for improved performance.

    • Main Results:

    • The novel method achieved sufficient limits of detection (LOD) and linear ranges for most analytes in complex samples.
    • Performance in retro-reflected beam interference detection surpassed previous reports.
    • Fluorescent sensitivity was comparable to conventional LIF systems, demonstrating a blend of high sensitivity and universal analysis.

    Conclusions:

    • The developed optical detection method offers a sensitive and versatile approach for capillary electrophoresis.
    • The combination of LIF and retro-reflected beam interference detection enhances analytical capabilities.
    • This technique shows promise for the analysis of various analytes, including fluorescent dyes, carbohydrates, and amino acids, without prior sample pretreatment.