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Biochemical characterization and tissue distribution of hamster complement C1s
H Sakiyama1, K Yamaguchi, K Chiba
1Division of Physiology and Pathology, National Institute of Radiological Sciences, Chiba-shi, Japan.
Journal of Immunology (Baltimore, Md. : 1950)
|January 1, 1991
Summary
Researchers developed monoclonal antibodies (mAbs) to study complement C1s. Hamster C1s are widely distributed in various tissues, indicating broad roles in hamster physiology.
Area of Science:
- Immunology
- Molecular Biology
- Histology
Background:
- Complement C1s is a crucial component of the classical complement pathway.
- Understanding the tissue distribution and synthesis of C1s is vital for elucidating its physiological functions.
Purpose of the Study:
- To generate monoclonal antibodies (mAbs) against hamster complement C1s.
- To investigate the localization and synthesis of C1s in hamster and rat tissues.
Main Methods:
- Production and characterization of mAbs (PG11, NG7, ED4) against hamster C1s.
- Immunohistochemical staining to determine C1s localization in tissue sections.
- RNA blot hybridization to confirm C1s synthesis.
- Immunoblot analysis of cell culture supernatants to assess C1s secretion.
Main Results:
- Monoclonal antibodies PG11 and NG7 cross-reacted with human and rat C1s.
- Hamster C1s exhibited widespread but specific staining in tissues derived from endoderm, mesoderm, and neuroectoderm.
- Strong C1s staining was observed in chondrocytes, stomach epithelium, intestinal epithelium, muscle cells, pia mater, choroid plexus epithelium, and hepatocytes.
- C1s synthesis was confirmed in various organs via RNA blot hybridization.
- Hepatocytes, kidney cells, and myoblasts secreted C1s into culture media.
Conclusions:
- Novel mAbs against hamster C1s were successfully generated.
- Hamster C1s are synthesized and secreted by multiple cell types and are localized in diverse tissues, suggesting broad involvement in immune and physiological processes.