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Updated: Jun 28, 2026
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Methylated DNA Immunoprecipitation
Published on: January 2, 2009
Methylated DNA immunoprecipitation (MeDIP).
Fabio Mohn1, Michael Weber, Dirk Schübeler
1Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
Methods in Molecular Biology (Clifton, N.J.)
|November 7, 2008
Summary
Methylated DNA immunoprecipitation (MeDIP) detects methylated DNA using antibodies against 5-methylcytidine. This method profiles DNA methylation genome-wide and identifies abnormal methylation in cancer.
Area of Science:
- Epigenetics
- Molecular Biology
- Genomics
Background:
- DNA methylation is a crucial epigenetic modification regulating gene expression.
- Accurate detection of DNA methylation patterns is essential for understanding cellular processes and disease.
- Existing methods may have limitations in unbiased, large-scale detection.
Purpose of the Study:
- To describe Methylated DNA Immunoprecipitation (MeDIP) as a versatile method for detecting methylated DNA.
- To highlight MeDIP's utility for both targeted and genome-wide DNA methylation profiling.
- To showcase MeDIP's application in identifying aberrant methylation in cancer.
Main Methods:
- Genomic DNA is sonicated to shear DNA fragments.
- Immunoprecipitation is performed using a monoclonal antibody specific to 5-methylcytidine.
- Enrichment of methylated DNA is assessed via PCR or genome-wide analysis with microarrays.
Main Results:
- MeDIP enables unbiased detection of methylated DNA.
- The protocol allows for assessment of methylation states in specific genomic regions.
- MeDIP coupled with microarrays generates comprehensive, genome-wide DNA methylation profiles.
Conclusions:
- MeDIP is a robust and adaptable technique for DNA methylation analysis.
- The method has been successfully applied in mammals and plants for large-scale profiling.
- MeDIP aids in the identification of differentially methylated genes, particularly in cancer research.
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