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Related Experiment Video

Updated: Jun 28, 2026

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

The MIRA method for DNA methylation analysis.

Tibor A Rauch1, Gerd P Pfeifer

  • 1Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 7, 2008
PubMed
Summary
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Aberrant DNA methylation patterns are hallmarks of human cancer. The methylated-CpG island recovery assay (MIRA) uses a protein complex to efficiently map these crucial DNA methylation patterns genome-wide.

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Cancer Research

Background:

  • DNA methylation alterations are common in human cancers.
  • Aberrant methylation is a key feature of cancer development.
  • Accurate methods are needed to study DNA methylation patterns.

Purpose of the Study:

  • To describe the methylated-CpG island recovery assay (MIRA).
  • To explain how MIRA identifies methylated DNA regions.
  • To demonstrate MIRA's application in genome-wide methylation mapping.

Main Methods:

  • Utilizes the MBD2b/MBD3L1 protein complex with high affinity for methylated DNA.
  • Employs the methylated-CpG island recovery assay (MIRA) for DNA enrichment.
  • Combines MIRA with microarray technology for genome-wide analysis.

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DNA Methylation: Bisulphite Modification and Analysis
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DNA Methylation: Bisulphite Modification and Analysis

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Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
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Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

Related Experiment Videos

Last Updated: Jun 28, 2026

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
13:11

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

Main Results:

  • MIRA effectively isolates CpG-methylated DNA.
  • The method enables the mapping of DNA methylation across the human genome.
  • Successful application of MIRA in conjunction with microarrays.

Conclusions:

  • MIRA is a valuable technique for characterizing DNA methylation.
  • This assay aids in understanding the role of aberrant methylation in cancer.
  • MIRA facilitates comprehensive genome-wide DNA methylation profiling.