Related Experiment Video

Updated: Jun 28, 2026

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
11:09

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay

Published on: September 11, 2016

An enterovirus epidemic in infants in the summer and fall of 2006

H F Trip1, D Schonenberg, J S Starreveld

  • 1Department of Pediatrics, Groene Hart Ziekenhuis, Gouda, The Netherlands.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
|November 7, 2008
PubMed

Insights

Diagnosing enterovirus in infants with fever requires testing multiple samples like stool and cerebrospinal fluid, as single-sample results are often incomplete. This comprehensive approach aids accurate patient management.

Area of Science:

  • Pediatrics
  • Infectious Diseases
  • Virology

Background:

  • Febrile illness in infants under 90 days necessitates thorough diagnostic evaluation.
  • Enterovirus infections are a significant concern in young infants, particularly during summer and fall.
  • Current diagnostic protocols may not fully capture the extent of enterovirus presence.

Purpose of the Study:

  • To assess the effectiveness of diagnostic procedures for enterovirus in infants with febrile illness.
  • To determine the optimal specimen types for enterovirus detection in this population.
  • To understand the impact of enterovirus diagnosis on infant patient management.

Main Methods:

  • Retrospective analysis of 24 infants younger than 90 days with febrile illness.
  • Sepsis evaluation including testing of stool and/or cerebrospinal fluid for enterovirus using polymerase chain reaction (PCR).
  • Comparison of diagnostic yields from single versus multiple specimen testing.

Main Results:

  • Enterovirus infection was diagnosed in 19 out of 24 infants (79%).
  • In cases where both stool and cerebrospinal fluid were tested, 33% (3/9) showed positive results in both specimens.
  • A significant proportion of infants received antibiotic treatment, even in the absence of bacterial infection.

Conclusions:

  • Comprehensive diagnostic evaluation for enterovirus, utilizing multiple specimen types, is crucial for accurate diagnosis in febrile infants.
  • Testing both stool and cerebrospinal fluid increases the likelihood of detecting enterovirus infection.
  • Timely and accurate enterovirus diagnosis influences clinical management decisions in infants.

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