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Related Experiment Video

Updated: Jun 28, 2026

Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber
27:58

Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber

Published on: October 1, 2007

Brain slice stimulation using a microfluidic network and standard perfusion chamber.

Javeed Shaikh Mohammed1, Hugo Caicedo, Christopher P Fall

  • 1Dept. of Bioengineering, University of Illinois, Chicago, IL, USA. javeed@uic.edu

Journal of Visualized Experiments : Jove
|November 8, 2008
PubMed
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We developed a novel two-level microfluidic device for electrophysiology. This device enables precise spatial and temporal control of chemical stimuli delivery to brain slices.

Area of Science:

  • Neuroscience
  • Bioengineering
  • Microfluidics

Background:

  • Electrophysiology studies require precise control over the chemical environment surrounding biological samples.
  • Existing methods for perfusing brain slices can be cumbersome and lack fine control over stimulus application.
  • Integration of microfluidic devices with electrophysiology setups offers a promising solution for enhanced experimental control.

Purpose of the Study:

  • To fabricate and characterize a novel two-level microfluidic device for seamless integration with electrophysiology systems.
  • To demonstrate the device's capability for precise spatial and temporal modulation of chemical stimuli applied to brain slices.
  • To enable advanced microscopy of brain slices during electrophysiological recordings.

Main Methods:

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Applying Microfluidics to Electrophysiology
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Applying Microfluidics to Electrophysiology

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Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity
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Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity

Published on: August 28, 2020

Related Experiment Videos

Last Updated: Jun 28, 2026

Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber
27:58

Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber

Published on: October 1, 2007

Applying Microfluidics to Electrophysiology
05:41

Applying Microfluidics to Electrophysiology

Published on: October 1, 2007

Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity
07:58

Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity

Published on: August 28, 2020

  • Fabrication of a two-level microfluidic device using a two-step standard negative resist lithography process.
  • Integration of the microfluidic device with off-the-shelf perfusion chambers for electrophysiology setups.
  • Utilizing a passive pumping method for fluid delivery and employing materials like glass coverslips and polydimethylsiloxane (PDMS) for optical transparency and biocompatibility.

    • Main Results:

    • Successful fabrication of a two-level microfluidic device with integrated microchannels and circular holes.
    • Demonstrated seamless integration with electrophysiology setups and perfusion chambers.
    • Showcased the device's ability to deliver spatially and temporally modulated chemical stimuli (neurotransmitters) to brain slices.
    • Confirmed compatibility with microscopy for observing brain slice dynamics.

    Conclusions:

    • The developed two-level microfluidic device offers a versatile platform for advanced neuroscience research.
    • It facilitates precise control over the microenvironment of brain slices, crucial for electrophysiology and imaging.
    • This technology enhances the ability to study neural function by enabling sophisticated chemical stimulation protocols.