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Related Experiment Video

Updated: Jun 28, 2026

Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device
12:00

Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device

Published on: October 1, 2007

Preparing e18 cortical rat neurons for compartmentalization in a microfluidic device.

Joseph Harris1, Hyuna Lee, Christina Tu Tu

  • 1Department of Biomedical Engineering, University of California, Irvine, CA, USA. jwharris7@gmail.com

Journal of Visualized Experiments : Jove
|November 8, 2008
PubMed
Summary

This video details the preparation of E18 cortical rat neurons, a crucial step for neuroscience research. The protocol ensures viable neurons for use in microfluidic devices.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Biotechnology

Background:

  • Primary neuronal cultures are essential for studying neuronal function and development.
  • Efficient and reproducible methods for preparing high-quality neurons are critical for experimental success.
  • E18 cortical rat neurons offer a well-established model system for various neurobiological investigations.

Purpose of the Study:

  • To provide a detailed protocol for the preparation of E18 cortical rat neurons.
  • To demonstrate the dissociation and purification techniques for obtaining viable individual neurons.
  • To prepare neurons for subsequent use in microfluidic devices for advanced research.

Main Methods:

  • Dissection of E18 fetal rat cortex.
  • Enzymatic dissociation using trypsin and mechanical trituration.

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Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons
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Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons

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Related Experiment Videos

Last Updated: Jun 28, 2026

Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device
12:00

Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device

Published on: October 1, 2007

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons
10:58

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons

Published on: August 22, 2007

Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons
10:50

Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons

Published on: November 2, 2018

  • Cell suspension in Neural Basal Media (NBM) with B27 and Glutamax.
  • Filtration through a 40 µm nylon cell strainer.
  • Centrifugation and re-suspension steps to optimize cell yield and viability.

    • Main Results:

    • Successful isolation of individual E18 cortical rat neurons.
    • Demonstration of a protocol amenable to preparing neurons for microfluidic applications.
    • Highlighting the importance of fresh tissue and optimized media for cell viability.

    Conclusions:

    • The presented method effectively prepares E18 cortical rat neurons for research applications.
    • This protocol serves as a valuable resource for neuroscientists requiring primary neuronal cultures.
    • The prepared neurons are suitable for immediate use in microfluidic systems, enabling advanced studies.