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Updated: Jun 28, 2026

Counting Human Neural Stem Cells
06:37

Counting Human Neural Stem Cells

Published on: August 22, 2007

Counting human neural stem cells.

Steven Marchenko1, Lisa Flanagan

  • 1Department of Pathology, University of California, Irvine, CA, USA.

Journal of Visualized Experiments : Jove
|November 8, 2008
PubMed
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This protocol details a quick hemacytometer method to count live cells using Trypan blue staining. It accurately quantifies cell concentration and total cell number for various cell types, including human neural stem/precursor cells.

Area of Science:

  • Cell Biology
  • Biotechnology

Background:

  • Accurate cell counting is crucial for reproducible cell culture.
  • Routine procedures like immunocytochemistry and cell transfections require precise cell numbers.

Purpose of the Study:

  • To present a simple, rapid protocol for live and dead cell differentiation.
  • To quantify cell concentration and total cell number using a hemacytometer.

Main Methods:

  • Detaching cells and resuspending in media.
  • Diluting cells with Trypan blue solution for hemacytometer loading.
  • Averaging viable cell counts across hemacytometer quadrants.

Main Results:

  • The method allows for differentiation between live and dead cells.

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Last Updated: Jun 28, 2026

Counting Human Neural Stem Cells
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Enumeration of Neural Stem Cells Using Clonal Assays

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Passaging Human Neural Stem Cells
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Passaging Human Neural Stem Cells

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  • Calculation yields precise cell concentration (cells per liter) and total cell number.
  • Applicable to human neural stem/precursor cells and other cell types.

    • Conclusions:

    • This hemacytometer-based protocol offers a fast and accurate way to determine cell viability and concentration.
    • The method is versatile and can be adapted for diverse cell culture applications.