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Related Experiment Video

Updated: Jun 28, 2026

Immunocytochemistry: Human Neural Stem Cells
19:41

Immunocytochemistry: Human Neural Stem Cells

Published on: August 24, 2007

Immunocytochemistry: human neural stem cells.

Steven Marchenko1, Lisa Flanagan

  • 1Department of Pathology, University of California, Irvine, CA, USA.

Journal of Visualized Experiments : Jove
|November 8, 2008
PubMed
Summary
This summary is machine-generated.

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This protocol details an immunocytochemistry method that conserves antibodies by using small coverslips and a novel staining technique. Researchers can obtain high-quality, reproducible data while reducing antibody usage for various cell types.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Immunology

Background:

  • Immunocytochemistry is vital for detecting antigens in cultured cells.
  • Optimizing antibody usage is crucial for cost-effectiveness and sustainability in research.

Purpose of the Study:

  • To present a streamlined immunocytochemistry protocol that minimizes antibody consumption.
  • To enable researchers to achieve high-quality, reproducible qualitative and quantitative staining data.

Main Methods:

  • Cells are cultured on small coverslips within tissue culture plates.
  • A novel staining method involves inverting coverslips onto minimal antibody drops on parafilm.
  • This technique reduces antibody solution needed per sample to approximately 25 microliters.

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Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
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Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Published on: December 18, 2014

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
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Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

Published on: January 28, 2015

Related Experiment Videos

Last Updated: Jun 28, 2026

Immunocytochemistry: Human Neural Stem Cells
19:41

Immunocytochemistry: Human Neural Stem Cells

Published on: August 24, 2007

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
13:05

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Published on: December 18, 2014

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
12:06

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

Published on: January 28, 2015

Main Results:

  • The protocol allows for significant conservation of primary and secondary antibodies.
  • High-quality, reproducible qualitative and quantitative data can be obtained.
  • The method is effective for human neural stem/precursor cells and adaptable to other cell types.

Conclusions:

  • This optimized immunocytochemistry protocol offers an efficient and cost-effective approach for cellular antigen analysis.
  • The method facilitates reproducible results with reduced antibody volumes, making it valuable for diverse research applications.