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A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

Published on: August 30, 2007

A microfluidic device with groove patterns for studying cellular behavior.

Bong Geun Chung¹, Amir Manbachi, Ali Khademhosseini

¹Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.

Journal of Visualized Experiments : Jove

|November 8, 2008

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This study presents a microfluidic device with microgrooved patterns for observing cellular behavior, specifically apoptosis. The device enables real-time monitoring of cellular responses to oxidative stress.

Area of Science:

Biotechnology
Cell Biology
Microfluidics

Background:

Microfluidic devices are valuable tools for studying cellular behavior.
Microgrooved substrates can influence cell alignment and function.
Observing cellular processes like apoptosis in real-time requires advanced imaging platforms.

Purpose of the Study:

To develop and characterize a novel microfluidic device with microgrooved patterns.
To investigate cellular responses, specifically apoptosis, using this microfluidic platform.
To assess the utility of the device for real-time cellular behavior studies and drug screening.

Main Methods:

Fabrication of a microfluidic device by bonding a poly(dimethylsiloxane) (PDMS) mold to a microgrooved substrate.
Immobilization and patterning of mouse fibroblast cells on microgrooved substrates of varying widths (25-100 microm).

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A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

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Perfusion of media containing hydrogen peroxide, Annexin V, and propidium iodide to induce and detect apoptosis.

Real-time observation and analysis of apoptosis using an inverted microscope with an incubation chamber.

Main Results:

The microfluidic device successfully patterned mouse fibroblast cells on microgrooved substrates.
Cells exposed to oxidative stress (hydrogen peroxide) exhibited apoptosis.
Annexin V staining confirmed the presence of apoptotic cells by detecting phosphatidylserine externalization.
Real-time monitoring of the apoptosis process was achieved within the microfluidic device.

Conclusions:

The developed microfluidic device with microgrooved patterns is effective for studying cellular behavior.
This platform facilitates real-time observation and analysis of cellular processes like apoptosis.
The device holds potential for high-throughput drug screening applications.