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Updated: Jun 28, 2026

A Gradient-generating Microfluidic Device for Cell Biology

A Gradient-generating Microfluidic Device for Cell Biology

Published on: August 30, 2007

A gradient-generating microfluidic device for cell biology.

Bong Geun Chung¹, Amir Manbachi, Wajeeh Saadi

¹Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.

Journal of Visualized Experiments : Jove

|November 8, 2008

Summary

This summary is machine-generated.

Researchers developed a microfluidic device to create stable growth factor gradients, observing fibroblasts migrating towards higher concentrations of epidermal growth factor (EGF). This tool aids in studying cell migration behavior.

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Last Updated: Jun 28, 2026

A Gradient-generating Microfluidic Device for Cell Biology

A Gradient-generating Microfluidic Device for Cell Biology

Published on: August 30, 2007

Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients

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Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Published on: April 4, 2013

Area of Science:

Biomedical Engineering
Cell Biology
Microfluidics

Background:

Microfluidic platforms offer precise fluid control for high-throughput experiments.
Poly(dimethylsiloxane) (PDMS)-based devices can generate stable, well-defined growth factor concentration gradients.

Purpose of the Study:

To fabricate and operate a novel gradient-generating microfluidic device.
To investigate cellular behavior, specifically fibroblast migration, in response to soluble concentration gradients.

Main Methods:

Fabrication of a simple microfluidic device with three inlets merging into one.
Gradient stability and profile confirmation using fluorescein isothiocyanate (FITC)-dextran.
Quantitative assessment of cell migration using cell tracking analysis.

Main Results:

The microfluidic device successfully generated stable concentration gradients.
Fibroblasts demonstrated directed migration towards higher concentrations of epidermal growth factor (EGF).
Cell migration directionality and motility were quantitatively analyzed.

Conclusions:

The developed microfluidic device is effective for generating stable gradients.
It provides a valuable tool for studying directed cell migration in response to growth factors.
This technology can advance the analysis of cellular behavior in controlled microenvironments.