Study on the spatial architecture of p53, MDM2, and p14ARF containing complexes
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Updated: Jun 28, 2026

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
Published on: September 28, 2012
Andrej Savchenko1, Mariya Yurchenko, Boris Snopok
1V. Lashkaryov Institute of Semiconductor Physics, NASU, 42 Prospekt Nauki, 03 028 Kyiv, Ukraine.
Abstract:
We have developed a surface plasmon resonance (SPR)-based immunocapture approach to study multimeric protein-protein complexes. A composition and spatial architecture of protein complexes that contained GST-tagged p53, p14ARF, and MDM2 was examined by the developed approach. Obtained results verified that the p53 protein possesses two binding sites for MDM2. Ternary complexes containing p14ARF, MDM2, and p53 proteins could only be formed when MDM2 protein functions as a bridging molecule. That was confirmed by immunoprecipitation and immunostaining.
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