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Related Experiment Videos

Vasopressin depolymerizes F-actin in toad bladder epithelial cells.

G H Ding1, N Franki, J Condeelis

  • 1Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.

The American Journal of Physiology
|January 1, 1991
PubMed
Summary
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Vasopressin (AVP) rapidly reduces F-actin in toad bladder cells, facilitating water channel vesicle fusion. This actin depolymerization is crucial for AVP

Area of Science:

  • Cell Biology
  • Physiology
  • Molecular Biology

Background:

  • Vasopressin (AVP) is a key hormone regulating water reabsorption in epithelial cells.
  • AVP's action involves the rapid fusion of intracellular vesicles containing water channels with the plasma membrane.
  • The precise molecular mechanisms, particularly cytoskeletal involvement, underlying this rapid fusion are not fully understood.

Purpose of the Study:

  • To quantitatively investigate the role of F-actin in vasopressin-stimulated water transport.
  • To determine the temporal relationship between AVP stimulation and changes in cellular F-actin content.
  • To explore the functional significance of F-actin dynamics in mediating vesicle fusion and water flow.

Main Methods:

  • Quantitative assessment of F-actin content in toad bladder epithelial cells using the rhodamine phalloidin binding assay.

Related Experiment Videos

  • Stimulation of cells with vasopressin (AVP), cyclic AMP analogs, and forskolin.
  • Measurement of water flow and F-actin levels following AVP washout, prostaglandin synthesis inhibition, and F-actin stabilization.

    • Main Results:

    • AVP stimulation caused a rapid and sustained reduction in cellular F-actin levels (15% reduction within 1 min, 20-30% reduction during AVP action).
    • Similar F-actin reductions were observed with cyclic AMP, a vasopressin analog, and forskolin.
    • F-actin levels increased above baseline after AVP washout; inhibiting prostaglandin synthesis enhanced water flow and F-actin decrease; F-actin stabilization inhibited water flow.

    Conclusions:

    • Vasopressin rapidly depolymerizes F-actin in target epithelial cells, shifting the equilibrium towards G-actin monomers.
    • This AVP-induced F-actin depolymerization is a critical and rapid event required for the fusion of water channel-containing vesicles.
    • The findings highlight the dynamic role of the actin cytoskeleton in regulating hormone-mediated membrane trafficking and transport.