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Upregulation of tumor necrosis factor receptor 1 and TNF-alpha converting enzyme during corneal wound healing

Tohru Sakimoto1,2, Ai Yamada3, Hitoshi Kanno4

  • 1Department of Ophthalmology, Nihon University School of Medicine, Tokyo, Japan. torusaki@med.nihon-u.ac.jp.

Japanese Journal of Ophthalmology
|November 11, 2008
PubMed
Abstract

Insights

Tumor necrosis factor-alpha converting enzyme (TACE) and tumor necrosis factor receptor-1 (TNFR1) are involved in corneal wound healing. TACE facilitates the release of soluble TNFR1 from fibroblasts and macrophages post-injury.

Area of Science:

  • Ophthalmology
  • Immunology
  • Cell Biology

Background:

  • Corneal wound healing is a complex process involving inflammatory mediators.
  • Tumor necrosis factor (TNF) signaling plays a role in tissue repair and inflammation.

Purpose of the Study:

  • To investigate the involvement of TNF-alpha converting enzyme (TACE) and TNF receptor-1 (TNFR1) in corneal wound healing.
  • To determine the expression patterns and functional roles of TACE and TNFR1 in the corneal healing process.

Main Methods:

  • Corneal alkali burns were induced in BALB/c mice.
  • TACE and TNFR1 expression was assessed using immunohistochemistry.
  • Soluble TNF-alpha (sTNF-alpha) and soluble TNFR1 (sTNFR1) release was measured in cultured human fibroblasts and mouse macrophages stimulated with phorbol myristate acetate (PMA).

Main Results:

  • TACE and TNFR1 expression were detected in stromal cells of alkali-burned corneas after the acute phase.
  • PMA stimulation promoted the release of both sTNF-alpha and sTNFR1 from macrophages.
  • PMA stimulation induced sTNFR1 release from fibroblasts, but not sTNF-alpha.

Conclusions:

  • TACE and TNFR1 are primarily expressed after the acute phase of corneal wound healing.
  • TACE mediates the extracellular release of sTNFR1 in both fibroblasts and macrophages.
  • These findings highlight the role of the TACE-TNFR1 axis in post-injury corneal tissue remodeling.

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