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Updated: Jun 28, 2026

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Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
Published on: March 6, 2013
Strategies for recombinant expression of small, highly disulphide-bonded, cationic antimicrobial peptides
A L Greenshields1, L C Knickle, R Syvitski
1Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada B3H 3Z1.
Protein and Peptide Letters
|November 11, 2008
Abstract:
Expression of two recombinant hepcidin homologues from Atlantic salmon, Salmo salar, characterization of their antimicrobial activity, and partial structural determination of the peptides is described. Expression was attempted in baculovirus and bacterial expression systems and the various purification and refolding methods used to determine the optimal strategy for production of active, correctly refolded hepcidin are reviewed.

