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Updated: Jun 28, 2026

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
Published on: February 1, 2011
A novel thermoacidophilic cellulase from Alicyclobacillus acidocaldarius
A Morana1, A Esposito, L Maurelli
1Institute of Protein Biochemistry - C.N.R., Via P. Castellino 111, 80131, Naples, Italy.
Abstract:
A novel cellulase was isolated from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius ATCC27009 grown in medium containing carboxymethylcellulose. The enzyme is a glycosylated monomer of 56.2 kDa, relatively thermostable, with optimal pH and temperature of 4.0 and 65 degrees C, respectively. Enzymatic assays on several polysaccharides demonstrated that CelG was specific for carboxymethylcellulose.
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