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High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

Cooperative binding of Drosophila heat shock factor to arrays of a conserved 5 bp unit

H Xiao1, O Perisic, J T Lis

  • 1Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853.

Cell
|February 8, 1991
PubMed

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Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
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