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Increased intestinal permeability in endotoxic pigs. Mesenteric hypoperfusion as an etiologic factor
M P Fink1, J B Antonsson, H L Wang
1Department of Surgery, University of Massachusetts Medical Center, Worcester 01655.
Archives of Surgery (Chicago, Ill. : 1960)
|February 1, 1991
Summary
Lipopolysaccharide (LPS) infusion in pigs increases gut mucosal permeability, but not solely due to reduced mesenteric blood flow. Other factors likely contribute to this LPS-induced permeability change.
Area of Science:
- Gastroenterology
- Physiology
- Immunology
Background:
- Lipopolysaccharide (LPS) infusion in pigs reduces superior mesenteric artery blood flow (Qsma).
- Mesenteric hypoperfusion is suspected to cause LPS-induced alterations in gut mucosal permeability.
Purpose of the Study:
- To investigate the role of mesenteric hypoperfusion in LPS-induced gut mucosal permeability changes.
- To differentiate the effects of LPS infusion from reduced blood flow on intestinal permeability.
Main Methods:
- Four groups of anesthetized swine were studied: LPS infusion with resuscitation, mechanical reduction of Qsma, ischemia/reperfusion model, and normal controls.
- Gut mucosal permeability was assessed by measuring plasma-to-lumen clearances of chromium 51-labeled edetic acid monohydrate (EDTA) and urea.
- The ratio of clearances (CEDTA/CUREA) was used to quantify permeability changes.
Main Results:
- LPS infusion (Group 1) resulted in a significant increase in CEDTA/CUREA (572% ± 235% of baseline).
- A severe ischemia/reperfusion model (Group 3) showed a marked increase in CEDTA/CUREA (999% ± 355% of baseline).
- Mechanical reduction of Qsma by 50% (Group 2) and control conditions (Group 4) did not significantly alter CEDTA/CUREA.
Conclusions:
- Increased gut mucosal permeability following LPS administration is not solely attributable to mesenteric hypoperfusion.
- Factors beyond reduced mesenteric blood flow contribute to LPS-induced increases in intestinal permeability.