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A simple method for the rapid removal of Bacillus anthracis spores from DNA preparations
Leslie A Dauphin1, Michael D Bowen
1Division of Bioterrorism Preparedness and Response (DBPR), National Center for Preparedness, Detection, and Control of Infectious Diseases (NCPDCID), CDC, Atlanta, Georgia, USA. Ldauphin@CDC.GOV
Abstract:
This study establishes a filtration method for the safe removal of Bacillus anthracis spores which may contaminate DNA preparations. Centrifugal filtration with 0.1-microm filter units can be used following extraction of DNA from B. anthracis spores to render samples safe without compromising the sensitivity of diagnostic real-time PCR assays for B. anthracis.
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