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Updated: Jun 28, 2026

Arteriovenous Metabolomics to Measure In Vivo Metabolite Exchange in Brown Adipose Tissue
Published on: October 6, 2023
Adipose tissue as a source of nicotinamide N-methyltransferase and homocysteine
Monika Riederer1, Wolfgang Erwa, Robert Zimmermann
1Institute of Molecular Biosciences, University of Graz, Heinrichstrasse 31A, A-8010 Graz, Austria. monika.riederer@uni-graz.at
Abstract:
Nicotinamide N-methyltransferase (NNMT) catalyses the conversion of nicotinamide to 1-methylnicotinamide and plays an important role in hepatic detoxification reactions. Here we show that, in addition to the liver, 3T3-L1 adipocytes as well as human and murine adipose tissue explants express high amounts of enzymatically active NNMT. NNMT mRNA levels and enzyme activity increased in 3T3-L1 cells in a differentiation-dependent manner. Homocysteine, the atherogenic product of the NNMT-catalyzed reaction, was secreted from 3T3-L1 cells or adipose tissue cultures. Homocysteine release increased during 3T3-L1 differentiation and was reduced when adipose tissue was treated with the NNMT inhibitor 1-methylnicotinamide. Nicotinic acid (NA), a widely used drug to lower elevated plasma lipid levels, induced NNMT enzyme activity in white adipose tissue of mice. In tissue culture nicotinamide treatment led to an increase in adipose tissue homocysteine secretion. These data support the concept that adipose tissue NNMT contributes to the increased plasma homocysteine levels in patients treated with NA.
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