Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Age but not sex modifies lymphoid immune responses in murine sepsis.

Research square·2026

Same author

SPAID: a comprehensive database for disease-specific autoantigens in autoimmune disorders.

Advanced biotechnology·2026

Same author

Sleep problems and emotional and behavioral problems among adolescents: examining independent types, cumulative scores, and latent patterns.

Child and adolescent psychiatry and mental health·2026

Same author

Large-scale association study identifies lung cancer susceptibility copy number variants and their potential functional role in genetic instability.

medRxiv : the preprint server for health sciences·2026

Same author

CN-RNN: a Deep Learning Framework for Copy Number Variation Detection with Exome Sequencing Data.

bioRxiv : the preprint server for biology·2026

Same author

The Effects of Digital Device Use on Psychological Health and Cognitive Functions in Chinese Older Adults.

International journal of geriatric psychiatry·2026

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

miRecords: an integrated resource for microRNA-target interactions.

Feifei Xiao¹, Zhixiang Zuo, Guoshuai Cai

¹Department of Neuroscience, University of Minnesota, Minneapolis, MN 55455, USA.

Nucleic Acids Research

|November 11, 2008

Summary

Researchers developed miRecords, a comprehensive database for animal microRNA (miRNA) target interactions. This resource includes validated and predicted interactions, aiding miRNA research and computational tool development.

More Related Videos

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Related Experiment Videos

Last Updated: Jun 28, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Area of Science:

Genomics
Bioinformatics
Molecular Biology

Background:

MicroRNAs (miRNAs) are key regulators of gene expression.
Computational prediction of miRNA targets is challenging.
Existing resources lack comprehensive, validated animal miRNA-target interaction data.

Purpose of the Study:

To develop miRecords, an integrated resource for animal miRNA-target interactions.
To provide a manually curated database of experimentally validated miRNA-target interactions.
To store predicted miRNA targets from established prediction programs.

Main Methods:

Manual curation of experimentally validated miRNA-target interactions.
Integration of data from 11 established miRNA target prediction programs.
Systematic documentation of experimental support for each interaction.

Main Results:

The Validated Targets component contains 1135 records of validated interactions.
These interactions involve 301 miRNAs and 902 target genes across seven animal species.
The Predicted Targets component aggregates predictions from 11 programs.

Conclusions:

miRecords serves as a valuable resource for experimental miRNA researchers.
The database supports the development of next-generation miRNA target prediction programs.
miRecords enhances the study of animal miRNA function and regulation.