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High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
INTERFEROME: the database of interferon regulated genes
Shamith A Samarajiwa1, Sam Forster, Katie Auchettl
1Center for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia.
Nucleic Acids Research
|November 11, 2008
Summary
INTERFEROME is a database of interferon-regulated genes, aiding research in infection, immunity, development, and cancer. It provides tools to identify interferon signatures and their biological impacts from gene expression data.
Area of Science:
- Immunology
- Genetics
- Bioinformatics
Background:
- Interferons (IFNs) are crucial cytokines involved in immune responses.
- Understanding IFN-regulated genes is vital for various research areas, including infection, immunity, development, and cancer.
- High-throughput expression technologies generate large datasets requiring specialized tools for analysis.
Purpose of the Study:
- To create an open-access database, INTERFEROME, for interferon-regulated genes.
- To integrate diverse biological information for comprehensive IFN research.
- To provide computational tools for identifying and analyzing interferon signatures.
Main Methods:
- Analysis of gene expression data from cells treated with IFNs.
- Integration of annotation, ontology, orthologue sequences (37 species), tissue expression, and gene regulatory information.
- Development of computational tools for signature identification.
Main Results:
- Established INTERFEROME, a comprehensive database of type I, II, and III IFN-regulated genes.
- Integrated multi-omics data for detailed investigation of IFN biology.
- Provided functional tools for high-throughput data analysis.
Conclusions:
- INTERFEROME serves as a valuable resource for researchers investigating IFN biology.
- The database facilitates the identification of interferon signatures and their consequences in various biological contexts.
- It supports advancements in infection, immunity, development, and cancer research.
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