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Related Experiment Video

Updated: Jun 28, 2026

Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex
09:45

Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex

Published on: March 28, 2012

NMDA receptor-dependent switching between different gamma rhythm-generating microcircuits in entorhinal cortex.

Steven Middleton1, Jozsi Jalics, Tilman Kispersky

  • 1Institute of Neuroscience, Newcastle University, Newcastle NE2 4HH, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America
|November 11, 2008
PubMed
Summary

The medial entorhinal cortex and hippocampus generate gamma rhythms independently. NMDA receptor blockade reveals a novel interneuron, the goblet cell, mediating distinct gamma frequencies crucial for memory and information transfer.

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Last Updated: Jun 28, 2026

Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex
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07:58

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Published on: August 28, 2020

Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice
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Published on: June 29, 2018

Area of Science:

  • Neuroscience
  • Computational Neuroscience

Background:

  • Local circuits in the medial entorhinal cortex (mEC) and hippocampus generate gamma frequency population rhythms independently.
  • Temporal interaction between these areas at gamma frequencies is implicated in memory, linked to NMDA-subtype glutamate receptor activity.

Purpose of the Study:

  • To investigate the role of NMDA receptors in mediating gamma rhythms in the mEC and hippocampus.
  • To identify the specific interneuron subtypes involved in different gamma frequency oscillations.

Main Methods:

  • Electrophysiological recordings in vivo and in vitro.
  • Computational modeling of neural circuits.
  • Pharmacological manipulation of NMDA receptors.

Main Results:

  • NMDA receptor blockade did not affect hippocampal gamma rhythm frequency but revealed a lower frequency (25-35 Hz) gamma rhythm in the mEC.
  • NMDA receptor-dependent mEC gamma rhythms were mediated by basket interneurons.
  • NMDA receptor-independent gamma rhythms were mediated by a novel interneuron subtype, the goblet cell, distinct from basket cells.

Conclusions:

  • Goblet cells represent a novel interneuron subtype mediating NMDA receptor-independent gamma rhythms in the mEC.
  • The two distinct gamma frequencies observed in mEC correlate with intrinsic frequencies in hippocampal areas CA3 and CA1.
  • NMDA receptor activation may control temporal interactions between mEC and hippocampus, influencing information transfer pathways critical for memory.