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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Interfacial Electrochemical Methods: Overview01:06

Interfacial Electrochemical Methods: Overview

Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current passing...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Chemical Ionization (CI) Mass Spectrometry01:21

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The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
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Updated: Jun 28, 2026

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
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Published on: April 17, 2017

Biphasic electrospray ionization for the study of interfacial complexes.

Michel Prudent1, Manuel A Méndez, Hubert H Girault

  • 1Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|November 11, 2008
PubMed
Summary

Biphasic electrospray ionization mass spectrometry enables studying interfacial complexation reactions. This technique provides insights into the stoichiometry of reactions involving lead ions and dipeptides with crown molecules.

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Determination of the Gas-phase Acidities of Oligopeptides
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Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
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Determination of the Gas-phase Acidities of Oligopeptides

Published on: June 24, 2013

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Mass spectrometry is a powerful analytical technique.
  • Electrospray ionization (ESI) is a common ionization method for mass spectrometry.
  • Biphasic electrospray ionization (BESI) is a novel variant of ESI.

Purpose of the Study:

  • To demonstrate the utility of BESI mass spectrometry for studying interfacial complexation reactions.
  • To investigate the complexation of aqueous lead ions by thioether crown molecules.
  • To analyze the complexation of an aqueous dipeptide by dibenzo-18-crown-6.

Main Methods:

  • Utilizing a dual-channel microsprayer for BESI.
  • Employing mass spectrometry to analyze interfacial complexation.
  • Studying reactions at the Taylor cone interface.

Main Results:

  • Successful implementation of BESI for studying interfacial complexation.
  • Characterization of lead ion complexation with thioether crown molecules.
  • Analysis of dipeptide complexation using dibenzo-18-crown-6 as an ionophore.
  • Mass spectra provided stoichiometric information on complexation reactions.

Conclusions:

  • BESI mass spectrometry is effective for studying interfacial complexation.
  • The technique offers valuable insights into reaction stoichiometry.
  • BESI has potential applications in chemical analysis and supramolecular chemistry.