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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Methods for Patch Clamp Capacitance Recordings from the Calyx
14:58

Methods for Patch Clamp Capacitance Recordings from the Calyx

Published on: July 29, 2007

Methods for patch clamp capacitance recordings from the calyx.

Kenneth Paradiso1, Wei Wu, Ling-Gang Wu

  • 1National Institute of Neurological Disorders and Stroke, National Institute of Health, USA. paradisk@ninds.nih.gov

Journal of Visualized Experiments : Jove
|November 11, 2008
PubMed
Summary
This summary is machine-generated.

This study details presynaptic patch clamp techniques at the calyx of Held, enabling measurement of vesicle release (exocytosis) and membrane recycling (endocytosis) in mammalian central nervous system synapses.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Electrophysiology

Background:

  • The calyx of Held is a large, easily identifiable synapse in the mammalian central nervous system.
  • Understanding presynaptic function, including vesicle dynamics, is crucial for comprehending synaptic transmission.

Purpose of the Study:

  • To present basic techniques for presynaptic patch clamp recording at the calyx of Held.
  • To demonstrate how capacitance measurements can quantify exocytosis and endocytosis at this synapse.

Main Methods:

  • Slice preparation of the calyx of Held.
  • Presynaptic patch clamp recording techniques.
  • Capacitance measurements to monitor membrane capacitance changes.

Main Results:

  • Successful electrical recordings from the presynaptic terminal.
  • Quantification of action potentials, calcium currents, and vesicle fusion (exocytosis).
  • Measurement of membrane uptake (endocytosis) via capacitance reduction.

Conclusions:

  • Presynaptic patch clamp and capacitance recording provide direct measures of exocytosis and endocytosis at the calyx of Held.
  • Simultaneous postsynaptic recordings are possible, offering a comprehensive view of synaptic activity.
  • This preparation is valuable for studying mammalian central nervous system synapse function.