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Updated: Jun 28, 2026

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Sensitive liposomes encoded with oligonucleotide amphiphiles: a biocompatible switch.

Arnaud Gissot¹, Carmelo Di Primo, Isabelle Bestel

¹Université de Bordeaux, 33076 Bordeaux Cedex, France.

Chemical Communications (Cambridge, England)

|November 11, 2008

Summary

DNA-tagged liposomes bind specifically to complementary DNA strands. External stimuli control liposome fluorescence by altering DNA probe location, enabling on/off switching for sensing applications.

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Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

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Area of Science:

Biotechnology
Nanotechnology
Molecular Biology

Background:

Liposomes are versatile nanocarriers with applications in drug delivery and diagnostics.
DNA-lipid interactions are crucial for developing novel biosensing platforms.
Controlling the localization and accessibility of DNA within liposomes is key for responsive systems.

Purpose of the Study:

To investigate the specific binding of DNA-tagged liposomes to complementary single-stranded DNA (ss-DNA).
To explore the influence of external stimuli on the fluorescence state of DNA-tagged liposomes.
To demonstrate the potential of these liposomes as responsive fluorescent probes.

Main Methods:

Fabrication of DNA-tagged liposomes using 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC).
Characterization of liposome binding to fluorescently labeled complementary ss-DNA.
Application of physical (temperature) and chemical (competitive ss-DNA sequences) stimuli.
Monitoring fluorescence changes indicative of probe localization (surface vs. bulk).

Main Results:

DNA-tagged DOPC liposomes exhibited specific binding to complementary ss-DNA without a linker.
Lipid bilayer composition had minimal impact on DNA binding specificity.
External stimuli (temperature, competitive DNA) induced reversible switching of liposome fluorescence.
Fluorescence switching correlated with the translocation of the DNA probe between the liposome surface and the bulk.

Conclusions:

DNA-tagged liposomes can be engineered for specific DNA recognition and responsive fluorescence.
Stimuli-responsive switching of liposome fluorescence is achievable by controlling DNA probe localization.
These findings pave the way for developing advanced DNA-based biosensors and smart drug delivery systems.