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Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
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Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

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Identifying transcriptional regulatory regions using reporter genes and DNA-protein interactions by chromatin

Lezanne Ooi1, Ian C Wood

  • 1Institute of Membrane and Systems Biology, University of Leeds, UK.

Methods in Molecular Biology (Clifton, N.J.)
|November 11, 2008
PubMed

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
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