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ATP-dependent leukotriene export from mastocytoma cells
T Schaub1, T Ishikawa, D Keppler
1Division of Tumor Biochemistry, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
FEBS Letters
|February 11, 1991
Summary
Leukotriene export from cells is an ATP-dependent process. This study identifies a novel ATP-dependent export carrier for leukotrienes (LT) in these cells.
Area of Science:
- Biochemistry
- Cell Biology
- Pharmacology
Background:
- Leukotrienes (LT) are crucial inflammatory mediators synthesized intracellularly.
- Following synthesis, LT are exported to the extracellular space to exert their functions.
Purpose of the Study:
- To characterize the mechanism of leukotriene export from cells.
- To identify the molecular nature of the leukotriene transporter.
Main Methods:
- Utilized plasma membrane vesicles from mastocytoma cells.
- Assessed ATP-dependent transport of various leukotrienes (LTC4, LTB4, LTD4, LTE4).
- Investigated inhibition by LTD4 receptor antagonists and a leukotriene biosynthesis inhibitor.
Main Results:
- Leukotriene export is an ATP-dependent primary active transport process.
- LTC4 showed the highest transport rate, with other LT derivatives transported at lower rates.
- LT transport was inhibited by specific LTD4 receptor antagonists and MK-886.
Conclusions:
- A novel ATP-dependent export carrier for leukotrienes exists in leukotriene-synthesizing cells.
- This carrier is a new member of the ATP-dependent exit pump family.
- Understanding this export mechanism could offer therapeutic targets for inflammatory diseases.