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Updated: Jun 28, 2026
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Diagnosis of Hirschsprung's Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5
Published on: April 26, 2019
[Neonatal diagnostic protocol of the Hirschsprung's disease]
I Tuduri Limousin1, J A Cabo Valdés, M J Moya Jiménez
1Servicio de Cirugía Pediátrica, Hospital Infantil, Hospitales Universitarios Virgen del Rocío, Sevilla.
Summary
This study introduces a diagnostic protocol using serial rectal manometry (RM) for Hirschsprung
Area of Science:
- Pediatric Surgery
- Gastroenterology
- Neonatal Diagnostics
Context:
- Hirschsprung's disease (HD) diagnosis typically relies on suction rectal biopsy (SRB), often delayed beyond the first month of life.
- Early diagnosis and management are crucial for neonates presenting with clinical symptoms suggestive of HD.
- Existing diagnostic methods may have limitations in early neonatal periods.
Purpose:
- To propose and evaluate a diagnostic protocol for Hirschsprung's disease (HD) in neonates.
- To enable timely management of HD by facilitating earlier diagnosis.
- To assess the efficacy and reliability of serial rectal manometry (RM) as a primary diagnostic tool.
Summary:
- A retrospective study analyzed 503 tests in 391 neonates suspected of HD, utilizing a protocol of serial weekly rectal manometry (RM).
- The protocol achieved a 99% sensitivity and a 0.9% false-negative rate for HD diagnosis.
- Sedation significantly improved study usefulness (2.97% vs. 8%) and reduced study time.
Impact:
- The proposed protocol allows for reliable diagnosis and management of Hirschsprung's disease in neonates.
- Implementing serial RM can overcome the age limitations of suction rectal biopsy (SRB).
- Sedation enhances diagnostic accuracy and efficiency in neonatal patients.
