Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Escherichia-Pseudomonas shuttle vectors derived from pUC18/19.

H P Schweizer1

  • 1Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences, Center, Canada.

Gene
|January 2, 1991
PubMed
Summary

Two novel plasmid vectors, pUCP18 and pUCP19, offer stable maintenance in Escherichia coli and Pseudomonas aeruginosa. These vectors provide convenient recombinant screening and versatile cloning for sequencing and expression applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Burkholderia pseudomallei colony variant necessary for gastric colonization.

mBio·2015
Same author

Down regulation of virulence factors of Pseudomonas aeruginosa by salicylic acid attenuates its virulence on Arabidopsis thaliana and Caenorhabditis elegans.

Infection and immunity·2005
Same author

Plant models for animal pathogenesis.

Cellular microbiology·2005
Same author

Small broad-host-range lacZ operon fusion vector with low background activity.

BioTechniques·2002
Same author

Vectors to express foreign genes and techniques to monitor gene expression in Pseudomonads.

Current opinion in biotechnology·2001
Same author

Vector design and development of host systems for Pseudomonas.

Genetic engineering·2001

Area of Science:

  • Molecular Biology
  • Microbiology
  • Genetics

Background:

  • Broad-host-range plasmids are essential tools for genetic manipulation in diverse bacterial species.
  • Existing vectors may have limitations in stability or functionality across different bacterial hosts.
  • Escherichia coli and Pseudomonas aeruginosa are key model organisms in microbiology research.

Purpose of the Study:

  • To construct and characterize two new broad-host-range plasmid vectors, pUCP18 and pUCP19.
  • To ensure stable maintenance of these vectors in both Escherichia coli and Pseudomonas aeruginosa.
  • To provide versatile tools for molecular cloning, sequencing, and protein expression in Gram-negative bacteria.

Main Methods:

  • Construction of pUCP18 and pUCP19 based on E. coli pUC18 and pUC19 vectors.

Related Experiment Videos

  • Assessment of plasmid stability in Escherichia coli and Pseudomonas aeruginosa.
  • Evaluation of features including recombinant screening, multiple cloning site versatility, and copy number.

    • Main Results:

    • Successfully constructed pUCP18 and pUCP19, demonstrating stable maintenance in both E. coli and P. aeruginosa.
    • Confirmed that the new vectors retain key features of pUC vectors, such as convenient screening and a versatile multiple cloning site.
    • The plasmids exhibit intermediate to high copy numbers, suitable for various molecular biology applications.

    Conclusions:

    • pUCP18 and pUCP19 are valuable additions to the molecular biologist's toolkit for Gram-negative bacteria.
    • These vectors offer a stable and versatile platform for genetic studies in E. coli and P. aeruginosa.
    • The design facilitates applications in DNA sequencing and heterologous protein expression.