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Comparison of pre-processing techniques for fluorescence microscopy images of cells labeled for actin

Gautam S Muralidhar1, Sumohana S Channappayya, John H Slater

  • 1University of Texas, Department of Biomedical Engineering, Austin, TX, USA.

AMIA ... Annual Symposium Proceedings. AMIA Symposium
|November 13, 2008
PubMed

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...