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Clathrin Coated Vesicles

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...

Pinching-off of Coated Vesicles

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Coat Assembly and GTPases

Coat Assembly and GTPases

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Mechanism of Lamellipodia Formation

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Intracellular Signaling Affects Focal Adhesions

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Related Experiment Video

Updated: Jun 28, 2026

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

Dab2 regulates clathrin assembly and cell spreading.

David Chetrit¹, Naomi Ziv, Marcelo Ehrlich

¹Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.

The Biochemical Journal

|November 13, 2008

Summary

This summary is machine-generated.

Disabled 2 (Dab2) protein, a clathrin adaptor, influences cell shape by regulating clathrin assembly dynamics. Dab2

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Last Updated: Jun 28, 2026

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

In vivo and in vitro Studies of Adaptor-clathrin Interaction

In vivo and in vitro Studies of Adaptor-clathrin Interaction

Published on: January 26, 2011

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Clathrin-mediated endocytosis is crucial for cellular processes.
Endocytic adaptors regulate clathrin assembly at the plasma membrane.
Dab2 (disabled 2) is a key adaptor protein involved in endocytosis.

Purpose of the Study:

To investigate the role of Dab2 isoforms and mutants in clathrin assembly.
To characterize the effect of Dab2 on clathrin-coated pit dynamics.
To explore the function of Dab2 in cell spreading and integrin-mediated adhesion.

Main Methods:

Utilized GFP (green fluorescent protein) fusion constructs of rat Dab2 isoforms and mutants.
Analyzed clathrin assembly size, distribution, and dynamics in COS7 cells.
Assessed cell spreading on fibronectin and integrin localization.

Main Results:

Overexpression of Dab2 p82 isoform enlarged clathrin assemblies at the plasma membrane.
Dab2-clathrin assemblies are dynamic and recruit other endocytic proteins (AP2, epsin).
Dab2's ability to induce enlarged assemblies and enhance cell spreading depends on its PTB domain, clathrin/phospholipid binding, and a poly-lysine stretch.

Conclusions:

Dab2 plays a significant role in regulating clathrin assembly dynamics.
The PTB domain, clathrin/phospholipid binding, and poly-lysine stretch are critical for Dab2 function.
Dab2 and clathrin are implicated in integrin-mediated cell spreading and focal adhesion formation.