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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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NDE1 and NDEL1: multimerisation, alternate splicing and DISC1 interaction.

Nicholas J Bradshaw1, Sheila Christie, Dinesh C Soares

  • 1The Centre for Molecular Medicine, Western General Hospital, The University of Edinburgh, Edinburgh EH4 2XU, UK.

Neuroscience Letters
|November 13, 2008
PubMed
Summary

Nuclear Distribution Factor E Homolog 1 (NDE1) and NDE-Like 1 (NDEL1) are homologous proteins. NDE1 interacts with DISC1 and itself, suggesting complex regulation of neuronal activity.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Nuclear Distribution Factor E Homolog 1 (NDE1) and NDE-Like 1 (NDEL1) are homologous mammalian proteins.
  • NDEL1 is extensively studied, but NDE1 remains poorly understood.
  • Disrupted in Schizophrenia 1 (DISC1) is implicated in neuronal development and function.

Purpose of the Study:

  • To investigate the isoforms and interactions of NDE1 in the brain.
  • To elucidate the relationship between NDE1, NDEL1, and DISC1.
  • To understand the complexity of DISC1-mediated regulation of neuronal activity.

Main Methods:

  • Western blotting to detect protein isoforms.
  • Co-immunoprecipitation assays to study protein-protein interactions.
  • Analysis of NDE1, NDEL1, and DISC1 interactions in brain tissue.

Main Results:

  • Multiple isoforms of NDE1 and NDEL1 were identified in the brain.
  • NDE1 directly binds to multiple DISC1 isoforms and to itself.
  • NDE1 forms complexes with NDEL1.

Conclusions:

  • NDE1 plays a significant role in neuronal function, interacting with DISC1 and NDEL1.
  • The interactions between NDE1, NDEL1, and DISC1 suggest a complex regulatory network.
  • Further research into NDE1 is crucial for understanding DISC1-mediated neuronal regulation.